Journal: Oncotarget
Article Title: Synergistic cytotoxic activity of cannabinoids from cannabis sativa against cutaneous T-cell lymphoma (CTCL) in-vitro and ex-vivo
doi: 10.18632/oncotarget.27528
Figure Lengend Snippet: PBL were isolated from blood samples of Sézary patients ( n = 6), and were treated with S4 (5 μg/mL), S5 (6 μg/mL), S4 (5 μg/mL) +S5 (6 μg/mL) and control (methanol; Vehicle treatment) for 48 h. Cells were harvested and analyzed by FACS following CD4-APC, CD26-alexa 405, Annexin V-FITC and PI staining. Apoptotic-induced cells (Annexin positive cells) were determined in the CD4 + CD26 - cell population and in non-CD4 + CD26 - cells of treated cells minus control. ( A ) The percent of apoptotic-induced CD4 + CD26 - cells is presented for single treatment compared to combined treatment; ( n = 4); *** denote significant difference between means (one way ANOVA; p < 0.001). ( B ) The percent of apoptotic-induced cells following the combined treatment was compared between CD4 + CD26 - cells and non-CD4 + CD26 - cells of SPBL; ( n = 6); ** denote significant difference between means (paired student T test; 0.001 < P < 0.05).
Article Snippet: Cells were suspended in 100 μL binding buffer with 1 μL Annexin V-FITC (4830-01-1, eBioscience) + 2 μL CD26 Alexa Fluor 405-conjugated antibody (FAB1180V-100UG, R&D SYSTEMS) + 10 μL CD4-APC-conjugated antibody (FAB3791A-100, R&D SYSTEMS) and incubated for 15 min at room temperature.
Techniques: Isolation, Control, Staining